Expression of miR-106a-5p, miR-106b-5p, and TGFβ1I1 in Peripheral Blood Mononuclear Cells (PBMCs) of Chemical Veterans Exposed to Sulfur Mustard With Long-term Pulmonary Complications

Background: Sulfur mustard as a chemical warfare agent causes short and long-term pulmonary complications in its victims. MicroRNAs are known to act remarkable regulators of biological pathways, monitoring, treatment diseases including respiratory problems. In this study, we investigated the expression miR-106a-5p miR-106b-5p, two TGF-β signaling, well their target molecule, TGFβ1I1, peripheral blood mononuclear cells from SM-exposed individuals. Materials Methods: A total 70 veterans with SM-induced were examined compared 35 gender age-matched healthy controls. After clinical examination function tests, severity was classified. Total RNA extracted PBMCs purity samples evaluated by NanoDrop 2000. The miR-106a-5p, TGFβ1I1 levels measured real-time RT-PCR. Results: significantly increased both mild (P=0.015) severe groups control group. miR-106b-5p considerably elevated group notably reduced Although, slight decrease observed control. Conclusion: Our results indicate that exposure sulfur affects gene, cells. Considering role regulation mentioned changes might point potential mechanism which SM chronic complications. ROC analysis, potentially turned out be suitable diagnostic biomarker categories patients. could considered better than miR-106b-5p.